Vol. XVIII · Free shipping $75+ · Read the collection
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Cell pellets were resuspended in lysis buffer [20 mM TrisHCl pH 8.0/0.5 M NaCl/10% (v/v) glycerol/1 mM phenylmethylsulfonylfluoride] and lysed using an ultra sonicator (Sonics TM Vibra Cell VCX 750

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